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<p class="MsoNormal"><o:p> </o:p></p>
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<span lang="EN-US" style="font-size:14.0pt;font-family:"Arial Narrow","sans-serif"">Vabilo na Preglov kolokvij / Invitation to the Pregl colloquium<o:p></o:p></span></p>
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<span lang="EN-US" style="font-family:"Arial Narrow","sans-serif""><o:p> </o:p></span></p>
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<span lang="EN-US" style="font-family:"Arial Narrow","sans-serif""><o:p> </o:p></span></p>
<p class="MsoNormal" align="center" style="margin-bottom:0cm;margin-bottom:.0001pt;text-align:center;line-height:normal;layout-grid-mode:char;text-autospace:none;vertical-align:baseline">
<b><span lang="EN-US" style="font-size:20.0pt;font-family:"Arial Narrow","sans-serif"">Prof. Dr. Ralf Wagner<o:p></o:p></span></b></p>
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<span lang="EN-US" style="font-size:14.0pt;font-family:"Arial Narrow","sans-serif"">Institute of Medical Microbiology and Hygiene, University Regensburg, Germany<o:p></o:p></span></p>
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<span lang="EN-US" style="font-size:14.0pt;font-family:"Arial Narrow","sans-serif"">Email:
</span><a href="mailto:ralf.wagner@ur.de"><span lang="EN-US" style="font-size:14.0pt;font-family:"Arial Narrow","sans-serif"">ralf.wagner@ur.de</span></a><span lang="EN-US" style="font-size:14.0pt;font-family:"Arial Narrow","sans-serif""><o:p></o:p></span></p>
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<span lang="EN-US" style="font-family:"Arial Narrow","sans-serif""><o:p> </o:p></span></p>
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<b><span lang="EN-US" style="font-size:20.0pt;font-family:"Arial Narrow","sans-serif"">Četrtek / Thursday 19. 1. 2017, ob / at 13:00
<o:p></o:p></span></b></p>
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<b><span lang="EN-US" style="font-size:16.0pt;font-family:"Arial Narrow","sans-serif""><o:p> </o:p></span></b></p>
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<span lang="EN-US" style="font-size:14.0pt;font-family:"Arial Narrow","sans-serif"">Velika predavalnica Kemijskega inštituta /
<o:p></o:p></span></p>
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<span lang="EN-US" style="font-size:14.0pt;font-family:"Arial Narrow","sans-serif"">Great Lecture Hall, National Institute of Chemistry;
<o:p></o:p></span></p>
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<span lang="EN-US" style="font-size:14.0pt;font-family:"Arial Narrow","sans-serif"">Hajdrihova 19, Ljubljana<b><o:p></o:p></b></span></p>
<p class="EndNoteBibliography" style="margin-bottom:0cm;margin-bottom:.0001pt"><span lang="EN-US" style="font-size:9.0pt;font-family:"Arial Narrow","sans-serif";color:#660066"><o:p> </o:p></span></p>
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<b><span lang="EN-US" style="font-size:16.0pt;font-family:"Arial Narrow","sans-serif";color:#792D4C"><o:p> </o:p></span></b></p>
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<b><span lang="EN-US" style="font-size:18.0pt;font-family:"Arial Narrow","sans-serif"">Synthetic biology toolbox supporting HIV vaccine development: from bench to bedside<o:p></o:p></span></b></p>
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<span lang="EN-US" style="font-size:14.0pt;font-family:"Arial Narrow","sans-serif";color:#792D4C"><o:p> </o:p></span></p>
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<span lang="EN-US" style="font-size:14.0pt;font-family:"Arial Narrow","sans-serif";color:black;mso-fareast-language:JA">Developing a safe and protective HIV vaccine is one of the most ambitious missions in current vaccinology. Here we show that by applying
principles of Synthetic Biology - knowledge-based in silico gene- and protein design, in vitro evolution technologies and antigen delivery via modified DNA- or poxviral vectors - we were able to come up with vaccine candidates that proved to induce immune
responses in HIV negative volunteers closely matching those responses seen in long term non-progressing individuals.<o:p></o:p></span></p>
<p class="MsoNormal" style="margin-bottom:5.0pt;text-align:justify;line-height:normal;layout-grid-mode:char">
<span lang="EN-US" style="font-size:14.0pt;font-family:"Arial Narrow","sans-serif";color:black;mso-fareast-language:JA">Knowledge-driven in silico design was initially based on the sequence of a high incidence HIV clade. Design steps towards improved T cell
immunogens comprised (i) selection and assembly of relevant epitopes supporting efficient cross presentation of immunogens to dendritic cells, (ii) RNA and codon optimization for maximal expression and (iii) exclusion of deleterious sequences and functional
protein domains imposing safety concerns. Such designer genes were synthesized cloned into a (i) DNA and (ii) a poxviral vector chassis for appropriate antigen delivery. B cell candidate vaccines are currently being engineered based on available structural
information of the viral envelope protein and selected using a recently developed cell display system, which takes advantage of a defined panel of broadly neutralizing antibodies.<o:p></o:p></span></p>
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<span lang="EN-US" style="font-size:14.0pt;font-family:"Arial Narrow","sans-serif";color:black;mso-fareast-language:JA">Preclinical and clinical data will be presented highlighting immunogenicity of the tested candidate vaccines.<o:p></o:p></span></p>
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<span lang="EN-US" style="font-size:14.0pt;font-family:"Arial Narrow","sans-serif""><o:p> </o:p></span></p>
<p class="MsoNormal" style="margin-bottom:0cm;margin-bottom:.0001pt;line-height:normal">
<span lang="EN-US" style="font-size:14.0pt;font-family:"Arial Narrow","sans-serif"">info:
<a href="mailto:roman.jerala@ki.si">roman.jerala@ki.si</a><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;line-height:115%;font-family:"Calibri","sans-serif""><o:p> </o:p></span></p>
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