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<p class="MsoNormal"><span style="font-size:12.0pt"><o:p> </o:p></span></p>
<p class="MsoTitle" style="margin:0cm;margin-bottom:.0001pt"><span style="font-size:18.0pt;font-family:"Verdana","sans-serif";color:black;font-weight:normal">VABILO NA PREGLOV KOLOKVIJ / INVITATION TO THE PREGL COLLOQUIUM<o:p></o:p></span></p>
<p class="MsoNormal"><b><span style="font-size:12.0pt;font-family:"Verdana","sans-serif""><o:p> </o:p></span></b></p>
<p class="MsoNormal" align="center" style="text-align:center;text-autospace:none">
<b><span style="font-size:18.0pt;font-family:"Verdana","sans-serif";mso-fareast-language:SL">Prof. dr. Alexander Kros<o:p></o:p></span></b></p>
<p class="MsoNormal" align="center" style="text-align:center;text-autospace:none">
<b><span style="font-size:8.0pt;font-family:"Verdana","sans-serif";mso-fareast-language:SL"><o:p> </o:p></span></b></p>
<p class="MsoHeader" align="center" style="text-align:center"><span lang="EN-GB" style="font-family:"Verdana","sans-serif"">Faculty of Science, Leiden Institute of Chemistry, Supramolecular & Biomaterials Chemistry<o:p></o:p></span></p>
<p class="MsoHeader" align="center" style="text-align:center"><span lang="EN-GB" style="font-family:"Verdana","sans-serif"">Gorlaeus Laboratories, Leiden; e-mail</span><span style="font-family:"Cambria","serif";color:black">:
</span><i><span lang="EN-GB" style="font-family:"Verdana","sans-serif""><a href="mailto:a.kros@chem.leidenuniv.nl"><span style="color:windowtext;text-decoration:none">a.kros@chem.leidenuniv.nl</span></a></span></i><span lang="EN-GB" style="font-family:"Verdana","sans-serif"">
<o:p></o:p></span></p>
<p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-GB" style="font-family:"Verdana","sans-serif""><o:p> </o:p></span></p>
<p class="MsoNormal" align="center" style="text-align:center"><span lang="EN-GB" style="font-family:"Verdana","sans-serif""><o:p> </o:p></span></p>
<p class="MsoNormal" align="center" style="text-align:center"><span lang="IT" style="font-size:8.0pt;font-family:"Verdana","sans-serif";mso-fareast-language:SL"><o:p> </o:p></span></p>
<p class="MsoNormal" align="center" style="text-align:center;line-height:150%"><b><span style="font-size:16.0pt;line-height:150%;font-family:"Verdana","sans-serif"">Četrtek / Thursday, 15. 01. 2015, ob / at 13:00<o:p></o:p></span></b></p>
<h6 style="mso-margin-top-alt:0cm;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm">
<b><span lang="FR" style="font-size:12.0pt;font-family:"Verdana","sans-serif"">Velika predavalnica Kemijskega inštituta / Lecture Hall at the National Institute of Chemistry; Hajdrihova 19, Ljubljana
<o:p></o:p></span></b></h6>
<p class="MsoNormal" style="mso-margin-top-alt:6.0pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:justify">
<b><span lang="EN-GB" style="font-size:9.0pt;font-family:"Verdana","sans-serif";color:#993366"><o:p> </o:p></span></b></p>
<p class="MsoNormal" align="center" style="text-align:center"><b><span lang="EN-GB" style="font-size:18.0pt;font-family:"Verdana","sans-serif";color:#993366">Understanding membrane fusion using a biomimetic model system<o:p></o:p></span></b></p>
<p class="MsoNormal" style="text-align:justify;text-indent:35.45pt"><span lang="FR" style="font-family:"Verdana","sans-serif""><o:p> </o:p></span></p>
<p class="MsoNormal" style="text-align:justify;text-indent:35.45pt"><span style="font-family:"Verdana","sans-serif"">Fusion of lipid bilayers, or membranes, is a ubiquitous process.
</span><span lang="EN-GB" style="font-family:"Verdana","sans-serif"">It occurs in the cells of our body during cargo exchange by membrane vesicles and during viral infection, but also in industrial processes such as yeast production. While many proteins like
SNAREs have been identified that play crucial roles in membrane fusion, the molecular mechanism of fusion remains unclear. This fascinating process is unexpectedly complex and our aim is to understand the details of this process using a biomimetic model system
which has all the characteristics of natural membrane fusion; </span><span style="font-family:"Verdana","sans-serif"">targeted docking, followed by lipid and content mixing in the absence of leakage</span><span lang="EN-GB" style="font-family:"Verdana","sans-serif"">.
<o:p></o:p></span></p>
<p class="MsoNormal" style="text-align:justify;text-indent:35.45pt"><span lang="EN-GB" style="font-family:"Verdana","sans-serif"">We developed a</span><span style="font-family:"Verdana","sans-serif""> model system composed of a complementary pair of lipidated
peptides able to form a heterodimeric coiled coil motif at the membrane interface similar to natural SNARE subunits.<sup>1,2,3</sup> The different steps of membrane fusion are currently studied using biophysical and biochemical techniques with a special focus
on peptide-peptide and peptide-lipid interactions. Unravelling</span><span lang="EN-GB" style="font-family:"Verdana","sans-serif""> of the molecular mechanism of membrane fusion using a multi-faceted approach of complementary techniques will result in a unique,
in depth view of the process of membrane fusion. <o:p></o:p></span></p>
<p class="MsoNormal" style="text-align:justify;text-indent:35.45pt"><span style="font-family:"Verdana","sans-serif"">To date, it is a major challenge to achieve efficient and targeted liposome delivery directly into the cytoplasm of live cells, circumventing
endocytotic pathways. In this respect, we recently studied and achieved membrane fusion between liposomes and live cells. Future</span><span lang="EN-GB" style="font-family:"Verdana","sans-serif""> applications are foreseen in drug delivery,</span><span style="font-family:"Verdana","sans-serif"">
nanoreactors</span><span lang="EN-GB" style="font-family:"Verdana","sans-serif""> and membrane engineering to name but a few.</span><span lang="FR" style="font-family:"Verdana","sans-serif""><o:p></o:p></span></p>
<p class="MsoNormal" style="text-align:justify;text-indent:35.45pt"><span style="font-family:"Verdana","sans-serif""><o:p> </o:p></span></p>
<p class="MsoNormal" style="text-align:justify"><span style="font-family:"Verdana","sans-serif"">1</span><span style="font-size:9.0pt;font-family:"Verdana","sans-serif"">) H. Robson Marsden, N.A. Elbers, P.H.H. Bomans, N.A.J.M. Sommerdijk, and
<b>A. Kros*</b>. A Reduced SNARE Model for Membrane Fusion. <i>Angewandte Chemie Int. Ed.</i>
<b>2009</b>, <i>48</i>, 2330–2333.<o:p></o:p></span></p>
<p class="MsoNormal" style="text-align:justify"><span lang="NL" style="font-size:9.0pt;font-family:"Verdana","sans-serif"">2) F. Versluis, J. Voskuhl, B. van Kolck, H. Zope, M. Bremmer, T. Albregtse and
<b>A. Kros*</b>. </span><span style="font-size:9.0pt;font-family:"Verdana","sans-serif"">Induced membrane fusion through in situ modification of plain liposomes with lipidated coiled coil forming peptides.
<i>J. Am. Chem. Soc.</i> <b>2013</b>, <i>135</i>, 8057–8062.<o:p></o:p></span></p>
<p class="MsoNormal" style="text-align:justify"><span lang="NL" style="font-size:9.0pt;font-family:"Verdana","sans-serif"">3) H.R. Zope, F. Versluis, A. Ordas, J. Voskuhl, H.P. Spaink and
<b>A. Kros*</b>. </span><i><span style="font-size:9.0pt;font-family:"Verdana","sans-serif"">In vitro</span></i><span style="font-size:9.0pt;font-family:"Verdana","sans-serif""> and
<i>In vivo</i> supramolecular biomembrane engineering using a lipidated coiled-coil motif.
<i>Angew. Chem. Int. Ed. Eng.</i> <b>2013</b>, <i>52(52)</i>, 14247-14251.<o:p></o:p></span></p>
<p class="MsoNormal" style="text-align:justify"><span style="font-size:9.0pt;font-family:"Verdana","sans-serif""><o:p> </o:p></span></p>
<p class="MsoNormal" align="center" style="text-align:center;line-height:150%"><b><span style="font-size:14.0pt;line-height:150%;font-family:"Verdana","sans-serif"">Vljudno vabljeni! / Kindly invited!<o:p></o:p></span></b></p>
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